Assessment of the real-time PCR and different digital PCR platforms for DNA quantification
نویسندگان
چکیده
منابع مشابه
Inter-laboratory assessment of different digital PCR platforms for quantification of human cytomegalovirus DNA
Quantitative PCR (qPCR) is an important tool in pathogen detection. However, the use of different qPCR components, calibration materials and DNA extraction methods reduces comparability between laboratories, which can result in false diagnosis and discrepancies in patient care. The wider establishment of a metrological framework for nucleic acid tests could improve the degree of standardisation...
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Real-time PCR continues to have a major impact across many disciplines of the biological sciences and this has been a driver to develop and improve existing instruments. From the first two commercial platforms introduced in the mid 1990s, there is now a choice in excess of a dozen instruments, which continues to increase. Advances include faster thermocycling times, higher throughput, flexibili...
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ژورنال
عنوان ژورنال: Analytical and Bioanalytical Chemistry
سال: 2015
ISSN: 1618-2642,1618-2650
DOI: 10.1007/s00216-015-9107-2